Dr. Bidyadhar Das

Zoology Department

Designation: Assistant Professor

Qualifications:

  • Ph. D. (2004) North-Eastern Hill University, Shillong-793022, Meghalaya
  • M. Sc. (1998) Berhampur University, Berhampur-760007, Odisha

Areas of Interest/Specialization: 

Characterization and regulation of metabolic enzymes / Cell Physiology and Biochemistry

Experience: 

Molecular Biology and Biochemistry
I have more than 12 years of teaching experience in Molecular Biology and Biochemistry at post-graduation level and more than 20 years of research experience. I obtained my Ph. D. degree working on the effect of isoflavones from Flemingia vestita on selected enzymes of carbohydrate metabolism in a cestode parasite. Further, I worked on the effect of isoflavones from F. vestita and Stephania glabra on the purified phosphoenolpyruvate carboxykinase (PEPCK) from the cestode parasite and also from human.

Presently, my research group and me are focussing on effect of genistein on AMPK-CRTC2 and MAPK-cJun/cFos signalling pathways responsible for PEPCK regulation, which may modulate hepatic gluconeogenesis in type 2 diabetes mellitus (T2DM). Further, we are working on the relationship of enzymes of carbohydrate- and lipid- metabolism in human, and plausible effect of phytochemicals on these enzymes in fighting T2DM.

Besides these, we are also focusing on regulation of Osteopontin expression via ERRβ and SIRT1) in highly metastasis breast cancer cell lines.

Research Projects

  • Evaluation of mitochondrial glycerophosphate dehydrogenase (mGPDH) for its role in type 2 diabetes mellitus (T2DM). (DST-SERB, Govt. of India: EMR/2016/001283 dated February 01, 2017)
  • Epigenetic status of pro/anti apoptotic activity of age-related genes involved in breast cancer. (DBT, Govt. of India: No. BT/253/NE/TBP/2011 dated May 07, 2012)
  • Analysis of molecular signalling proteins and epigenetic factors responsible for regulation of PEPCK expression. (DBT, Govt. of India: No. BT/103/NE/TBP/2010 dated March 16, 2011)
  • Comparative Proteomic and transcriptomic analyses of PEPCK under the influence of Flemingia vestita-derived phytoestrogen (Genistein). (DBT, Govt. of India: No. BT/PR12828/Med/30/221/2009 dated March 22, 2010)
  • Bioprospection for anthelmintic efficacy of some medicinal plants used in traditional medicine system in North-east India. (DST-SERC, Govt. of India: No. SR/FT/L-107 /2004 dated May 04, 2005)

Publications

 22 research articles, 1 review article, and 3 book chapters  (*corresponding author)

  • Pasupuleti B.G., Khongsti K., Das B.*, Bez G.* (2020) 1,2,3-Triazole tethered 1,2,4-trioxanes: Studies on their synthesis and effect on osteopontin expression in MDA-MB-435 breast cancer cells. European Journal of Medicinal Chemistry 186:111908 DOI 10.1016/j.ejmech.2019.111908
  • Tarianga K., Ramanujam S.N., Das B.* (2019) Effect of arsenic (As) and lead (Pb) on glycogen content and on the activities of selected enzymes involved in carbohydrate metabolism in freshwater catfish, Heteropneustes fossilis. International Aquatic Research 11:253-266 DOI: 10.1007/s40071-019-00234-2
  • Tandon V., Das B.* (2018) Genistein: Is the multifarious botanical a natural anthelmintic too? Journal of Parasitic Diseases 42:151-161 DOI:10.1007/s12639-018-0984-0
  • Dkhar B., Khongsti K., Thabah D., Syiem D., Satyamoorthy K., Das B.* (2018) Genistein represses PEPCK-C expression in an insulin-independent manner in HepG2 cells and in alloxan-induced diabetic mice. Journal of Cellular Biochemistry 119:1953-1970 DOI:10.1002/jcb.26356
  • Ramnath, Dutta A.K., Dkhar B., Tandon V., Das B.* (2017) Biological significance of phosphoenolpyruvate carboxykinase in a cestode parasite, Raillietina echinobothrida, and effect of phytoestrogens on the enzyme from the parasite and its host, Gallus domesticus. Parasitology 144:1264-1274 doi:10.1017/S0031182017000518
  • Dutta A.K., Ramnath, Dkhar B., Tandon V., Das B.* (2016) Cloning and expression of phosphoenolpyruvate carboxykinase from a cestode parasite and its solubilization from inclusion bodies using L-arginine. Protein Expression and Purification 125:61-67 DOI: http://dx.doi.org/10.1016/j.pep.2015.09.004
  • Seenappa V., Das B., Joshi B.M., Satyamoorthy K.* (2016) Context dependent regulation of PEPCK isoforms via promoter DNA methylation and RNA stability in human cells. Journal of Cellular Biochemistry 117:2506-2520. doi: 10.1002/jcb.25543
  • Dutta A.K., Ramnath, V. Tandon, Das B.* (2016) Biocomputational analysis of phosphoenolpyruvate carboxykinase from Raillietina echinobothrida, a cestode parasite, and its interaction with possible modulators. Parasitology 143:300-313 doi:10.1017/S0031182015001742
  • Das B., Ramnath, Dutta A.K., Tandon V*. (2015) Differential kinetics at PK/PEPCK branch point in the cestode, Raillietina echinobothrida. Experimental Parasitology 153:151-159 DOI: 10.1016/j.exppara.2015.03.023
  • Ramnath, Jyrwa D.B., Dutta A.K., Das B., Tandon V.* (2014) Molecular characterization of the Indian poultry nodular tapeworm, Raillietina echinobothrida (Cestoda: Cyclophyllidea: Davaineidae) based on rDNA Internal Transcribed spacer 2 region. Journal of Parasitic Diseases 38:22-26 DOI: 10.1007/s12639-012-0184-2
  • Das B., Tandon V.*, Saxena J.K., Joshi S., Singh A.R. (2013) Purification and characterization of phosphoenolpyruvate carboxykinase from Raillietina echinobothrida, a cestode parasite of the domestic fowl. Parasitology 140:136–146 doi:10.1017/S0031182012001254
  • Das B., Tandon V.*, Lyndem L.M., Gray A.I., Ferro V.A. (2009) Phytochemicals from Flemingia vestita (Fabaceae) and Stephania glabra (Menispermeaceae) alter cGMP concentration in the cestode Raillietina echinobothrida. Comparative Biochemistry and Physiology Part C 149:397-403
  • Lyndem L.M., Tandon V.*, Das B. (2008) Anthelmintic efficacy of medicinal plants from Northeast India against hookworms: an in vitro study on Ancylostoma ceylanicum. Pharmacologyonline 3: 697-707
  • Das B., Tandon V.*, Saha N. (2007) Genistein from Flemingia vestita (Fabaceae) enhances NO and its mediator (cGMP) production in a cestode parasite, Raillietina echinobothrida. Parasitology 134: 1457-1463
  • Tandon V., Das B.* (2007) In vitro testing of anthelmintic efficacy of Flemingia vestita (Fabaceae) on carbohydrate metabolism in Raillietina echinobothrida. Methods 42:330-338
  • Das B., Tandon V.*, Saha N. (2006) Effect of isoflavone from Flemingia vestita (Fabaceae) on the Ca2+ homeostasis in Raillietina echinobothrida, the cestode of domestic fowl. Parasitology International 55:17-21
  • Tandon V.*, Chakravarty R, Das B. (2005) Four new species of the genus Lytocestus (Caryophyllidea, Lytocestidae) from Edible Catfishes in Assam and Meghalaya, India. Journal of Parasitic Diseases 29:131-142
  • Tandon V.*, Kar P.K., Das B., Sharma B., Dorjee J. (2005) Preliminary survey of gastro-intestinal helminth infection in herbivorous livestock of mountainous regions of Bhutan and Arunachal Pradesh. Zoos’ Print Journal 20:1867-1868
  • Das B., Tandon V.*, Saha N. (2004) Effects of phytochemicals of Flemingia vestita (Fabaceae) on glucose 6-phosphate dehydrogenase and enzymes of gluconeogenesis in a cestode (Raillietina echinobothrida). Comparative Biochemistry and Physiology Part C 139:141-146
  • Tandon V.*, Lyndem L.M., Kar P.K., Pal P., Das B., Rao H.S.P. (2004) Anthelmintic efficacy of rhizome-pulp extract of Stephania glabra and aerial root extract of Trichosanthes multiloba in vitro: two indigenous plants in Shillong, India. Journal of Parasitic Diseases 28:37-44
  • Das B., Tandon V.* and Saha N. (2004) Anthelmintic efficacy of Flemingia vestita (Fabaceae): alterations in glucose metabolism of the cestode, Raillietina echinobothrida. Parasitology International 53:345-350
  • Das B., Tandon V.* and Saha N. (2004) Anthelmintic efficacy of Flemingia vestita (Fabaceae): alteration in the activities of some glycolytic enzymes in the cestode, Raillietina echinobothrida. Parasitology Research 93:253-261
  • Tandon V.*, Das B. and Saha N. (2003) Anthelmintic efficacy of Flemingia vestita (Fabaceae): effect of genistein on glycogen metabolism in the cestode, Raillietina echinobothrida. Parasitology International 52:179-183

Chapters in Books

  • Das B.* and Dutta A. (2018) Phosphoenolpyruvate carboxykinase: a target enzyme in management of helminth parasites? In Advances in medico-veterinary parasitology: an Indian perspective (Eds: Yadav A.K., Tandon V. and Hoti S. L.). 459-474
  • Tandon V., Das B.* and Kumar S. (2017) Proteases of parasitic helminths: their metabolic role in establishment of infection in the host. In Proteases in Human Disease  (Eds: Chakraborti S.,  Chakraborti T.  and Dhalla N. S.). 247-262 DOI 10.1007/978-981-10-3162-5_12
  • Tandon V.*, Yadav A. K., Roy B. and Das B. (2011) Phytochemicals as cure of worm infections in traditional medicine systems. In Emerging Trends in Zoology (Eds: Srivastava U. C. and Kumar S.). 351-378

Awards

  • DBT Overseas associateship award to University of Waterloo, Canada (2015-2016)
  • DBT Overseas associateship award to Strathclyde University, Scotland (2012-2013)
  • National Eligible Test (CSIR-UGC) 2000, Life Sciences
  • Graduated Aptitude Test in Engineering 1998, IIT, Delhi, Life Sciences


Ph. D. Awarded/Supervised:

  • Ms. Riva Mary Rani (yet to be registered)
  • Mr. Superior Syngkli (yet to be registered)
  • Mr. Kermi-u Tariang (2453 of 08/12/2014)
  • Mr. Kitboklang Khongsti (2251 of 11/12/2013)
  • Dr. Barilin Dkhar (1878 of 20/09/2011) awarded on 10/05/2018
  • Dr. Asim K. Dutta (1738 of 21/04/2011) awarded on 20/12/2016
  • Dr. Ramnath (1627 of 17/09/2010) awarded on 28/10/2016

Administrative responsibilities undertaken

  • HOD-in-charge, Department of Zoology, NEHU
  • Member of Board of School of Life Sciences, NEHU
  • Member of Board of Studies, Department of Zoology, NEHU
  • Member of M. Sc. and Ph. D. admission committee, Department of Zoology, NEHU
  • Presiding officer for NEHUSU election
  • Member of Counselling and Placement Cell, NEHU

Academic affiliations 

  • Member (57681M) of American Society for Biochemistry and Molecular Biology, U.S.A.
  • Life membership (L/124/2015) of Society for Mitochondrial Research and Medicine, India
  • Life membership (LM 699 of 2005) of Indian Society for Parasitology, India


Papers presented in Conferences/Seminars:

  • “Genistein: Is the multifarious botanical a natural anthelmintic too?” at “14th International Congress of Parasitology (ICOPA 2018)” held at Daegu, Republic of Korea, during 19th August 2018 - 24th August 2018
  • “Regulation of OPN expression in MDA-MB-435: role of genistein” at EXPERIMENTAL BIOLOGY 2018 held at San Diego Convention Center, 111 West Harbor Drive, San Diego, California, USA, during 21st April 2018 – 25th April 2018
  • “Cloning and characterization of phosphoenolpyruvate carboxykinase in Raillietina echinobothrida (Cestoda: Cyclophyllidea)” in Global Challenges in the Management of Parasitic Diseases conducted by Indian Society of Parasitology and CDRI, held at Lucknow Oct 16-18, 2014
  • “Genistein, an isoflavone from Flemingia vestita, in anthelmintic role” in the 76th Annual Session of the National Academy of Sciences, India’ held at Indian Institution of Technology Bombay, Mumbai, 6th-8th October 2006
  • “Effect of isoflavone from Flemingia vestita (Fabaceae) on the cGMP accumulation in Raillietina echinobothrida” in the XIth International Congress of Parasitology held at Glasgow, Scotland, 6th-11th August 2006
  • “Prevalence of zoonotic helminths and traditional remedies against helminthiasis in Meghalaya, North-east India.” in the ‘XII General Assembly, Scientific Committee on the Problems of the Environment’ held at New Delhi, 7th-11th, February 2005, sponsored by INSA and NCPGR, Govt. of India
  • “Effect of isoflavone from Flemingia vestita (Fabaceae) on the Ca2+ homeostasis in Raillietina echinobothrida, the cestode of domestic fowl.” in the 17th National Congress of Parasitology held at Regional Medical Research Center, Dibrugarh, 24th-26th October 2005
  • “Anthelmintic efficacy of Flemingia vestita (Fabaceae): alteration in the activities of some enzymes of glycolysis in the cestode, Raillietina echinobothrida.” in the 3rd Global Meet on Parasitic Diseases’ held at Bangalore University, Bangalore, 12th-16th January 2004, organized by the Bangalore University, Indian Society for Parasitology and Society for Applied Genetics, Bangalore University
  • “Anthelmintic efficacy of Stephania glabra and Trichosanthes multiloba against hookworms: an in- vitro study on Ancylostoma ceylanicum.” in the ‘3rd Global Meet on Parasitic Diseases’ held at Bangalore University, Bangalore, 12th-16th January 2004
  • “Anthelmintic efficacy of Flemingia vestita (Fabaceae): effect of genistein on glycogen metabolism in the cestode, Raillietina echinobothrida” in 15th National Congress of Parasitology held at Desert Regional Station, ZSI, Jodhpur, 1st-3rd October 2001 sponsored by Indian Society for Parasitology

Collaboration/Consultancy

  • Dr. Todd Holyoak, University of Waterloo, ON N2L 3G1, Canada
  • Dr. V. Ferro, SIPBS, Strathclyde University, Scotland, UK
  • Dr. S. Mishra, Scientist-F, ILS, Bhubaneswar, Odisha
  • Prof. K. Satyamoorthy, Director, MLSC, Manipal University, Manipal
  • Dr. A. Kataki, Director, BBCI, Guwahati, Assam

Any other:

i) External reviewer for National and International Journals

  • Biochemical Journal
  • Diabetes, Obesity and Metabolism
  • The Journal of Nutritional Biochemistry
  • PlosOne
  • Bioscience Reports
  • Comparative Biochemistry and Physiology Part B
  • Molecular and Biochemical Parasitology
  • Veterinary Parasitology
  • Biochemistry and Biophysics Reports
  • Journal of Parasitic Diseases

ii) Other academic activities

  • External examiner in Visva-Bharati University, Santiniketan
  • Ph. D. evaluator (external examiner) in SRM University, Chennai
  • Coordinator in Special Summer School in Basic Sciences and Technology 2019 for Universities and College teachers
  • Resource person in refresher courses for Universities and College teachers
  • Organized “2nd Colloquium of DBT Overseas Associateship meeting (2013) at NEHU